Animal physiology
mokhtar fathi
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
A study with 160 one-day age male broilers (Ross 308) was for six week period, conducted to investigate the some blood parameters, and plasma thyroxin (T4) and triiodothyronine (T3) of broiler chickens with cold induced pulmonary hypertension syndrome (PHS). Chicks in 2 groups of 4 replicates were studied ...
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A study with 160 one-day age male broilers (Ross 308) was for six week period, conducted to investigate the some blood parameters, and plasma thyroxin (T4) and triiodothyronine (T3) of broiler chickens with cold induced pulmonary hypertension syndrome (PHS). Chicks in 2 groups of 4 replicates were studied in each group20 chicks for each replicate were used. One group set as control (healthy) group and reared under normal temperature, second group set as PHS group and reared under cold temperature. At the end of the experiment (day42), 2 chicks from each replicate were randomly selected bled and slaughtered. The heart was removed and the right ventricle was dissected away. The ratio of right ventricle weight to total ventricle weight (RV/TV) was calculated. The results showed that hematocrit value, mortality due to ascites, RV/TV index and differential white blood cells count were significantly higher in CT treatment chick (P˂0.05). The concentrations of plasma t4 of CT birds were significantly lower than other group birds at day 21 & 42. it is also, The concentrations of plasma t3 of CT birds were significantly higher than other group birds at day 21(exception day 42) (P˂0.05).
Mokhtar Fathi; Mohamad Heydari; Mehran Mohamadikhah
Abstract
The present study was performed to investigate the effect of furosemide on oxidative stress, mortality due to ascites and some blood parameters in broiler chickens. A total of 450 Ross 308 days-old chicks were assigned to 3 groups (control and two treatments of 30 and 60 ppm zamide) in a completely randomized ...
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The present study was performed to investigate the effect of furosemide on oxidative stress, mortality due to ascites and some blood parameters in broiler chickens. A total of 450 Ross 308 days-old chicks were assigned to 3 groups (control and two treatments of 30 and 60 ppm zamide) in a completely randomized design with 5 replications and 30 chicks per replicate. The birds were subjected to a special cold temperature program to induce ascites. Blood parameters: red blood cell count, hematocrit, hemoglobin, white blood cells, activity of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, creatine kinase and oxidative stress parameters such as; Plasma antioxidant capacity, plasma malondialdehyde, glutathione peroxidase and superoxide dismutase activity, glucose, protein, triglyceride, cholesterol and high-density lipoprotein were measured. Performance parameters of body weight gain, feed intake and feed conversion ratio were also evaluated. Deaths were recorded daily and described to differentiate the cause of death and determine ascites. The results showed that administration of 60 ppm of furosemide significantly reduced weight and feed intake as well as improved feed conversion ratio (P<0.05). In addition, furosemide, significantly decreased ascites index and ascites losses (P<0.05). Also, level of 60 ppm furosemide, increased white blood cells, plasma antioxidant capacity, glutathione-peroxidase activity). Decreased plasma protein, malondialdehyde and activity of plasma aspartate, aminotransferase, alkaline phosphatase, and creatinine kinase (P<0.05). The results of this research show that the level of 60 ppm Furosemide can reduce the problems caused by high blood pressure in birds with this metabolic syndrome by improving its antioxidant status.
Mokhtar Fathi; Shahriyar Saeidian
Abstract
Regarding the role of arginine in vasodilatation and reduction of blood pressure and the possibility of replacing this amino acid with guanidinoacetic acid, a test was conducted with 400 chicks in 5 treatments (control, two levels of 0.5 and 1% arginine, and two levels of 0.15 And 0.3% Guanidininostech) ...
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Regarding the role of arginine in vasodilatation and reduction of blood pressure and the possibility of replacing this amino acid with guanidinoacetic acid, a test was conducted with 400 chicks in 5 treatments (control, two levels of 0.5 and 1% arginine, and two levels of 0.15 And 0.3% Guanidininostech) in four replications in a completely randomized design. Birds were subjected to a cold temperature program for induction of pulmonary hypertension syndrome. Blood parameters (RBC, hemoglobin, hematocrit and heterophile / lymphocyte), biochemical parameters (lactate, urea, uric acid and nitric oxide), enzymatic parameters (LDH, AST, ALT, CK) and antioxidant TAS, MDA, GPX, SOD) in plasma were measured at the end of the experiment (day 42). The results showed that supplementation of 1% arginine and 0.15% guanidinoacetic acid significantly decreased the heterophilic/ lymphocyte index, hematocrit, urea and uric acid, and increased nitric oxide (P <0.05). Treatment with 1% arginine and 0.15% guanidinoacetic acid reduced malondialdehyde and increased activity of superoxide dismutase and creatine kinase in plasma (P <0.05). The level of 1% of arginine also significantly increased the activity of glutathione peroxidase enzyme in the plasma. Other measured parameters were not significantly affected by experimental treatments (P> 0.05). In general, the results of this experiment showed that, given the lower price of guanidinoacetic acid compared to arginine, it is possible to effectively use 0.15% guanidinoacetic acid to replace arginine supplementation in preventing reduce stress.
Shahriar Saeidian; Zahra Baghaei Far; Zivar Parvanak; Mokhtar Fathi
Abstract
Breast cancer accounts for about 10% of all cancers in the world and accounts for 30% of all cancers in women. Therefore, its early detection will play an important role in its treatment. Because lncRNAs are expressed differently in cancerous tissues than in normal tissues, they increase the potential ...
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Breast cancer accounts for about 10% of all cancers in the world and accounts for 30% of all cancers in women. Therefore, its early detection will play an important role in its treatment. Because lncRNAs are expressed differently in cancerous tissues than in normal tissues, they increase the potential of these molecules as biomarkers for disease diagnosis. Also, changes in the expression of lncRNAs in patients with different types of cancer subtypes and different races intensify the importance of using these molecules as biomarkers for disease diagnosis. Therefore, the aim of this study was to investigate the expression of GAS5, NEAT1 and SRA lncRNAs in cancer specimens with cancer and in healthy individuals. In this study, from the tumor tissue of 22 patients with breast cancer and also 22 samples of healthy tissue from individuals under the direct supervision of a pathologist and according to clinical signs and laboratory findings were collected from hospitals in Isfahan. After RNA extraction from tumor and normal tissue, cDNA was fabricated according to RT-qPCR method. The lncRNA expression level of GAS5, NEAT1 and SRA genes was calculated by ∆∆CT method. The expression pattern was analyzed using Rest 2009 software as well as SPSS version 16. Real Time Reverse transcription-PCR results showed that the mean relative gene expression in tumor samples was significantly lower for GAS5 and NEAT1 lncRNAs than normal samples. But no expression change was observed for lncRNA SRA.
Mokhtar Fathi; Taimour Tanha; Naji Ahmadi
Abstract
This experiment was conducted to investigate the effects of different levels of prebiotic (Fermacto) on performance and some blood parameters of broiler chicks under induced ascites. 240-old chicks from Ross 308 strain were randomly divided into three treatments and each treatment was divided into four ...
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This experiment was conducted to investigate the effects of different levels of prebiotic (Fermacto) on performance and some blood parameters of broiler chicks under induced ascites. 240-old chicks from Ross 308 strain were randomly divided into three treatments and each treatment was divided into four replicates. The dietary treatments included: control and two levels (2 and 4 g/kg feed) of prebiotic (Fermacto). For ascites induction, all birds received 1.5 grams salt per liter from day 6 to day 13. The relative weight of the intestines was measured relative to the body weight at end of experiment. The birds of the negative treatments were subjected to an induction program. Prebiotic supplements were added to the feed from the 12th day of the experiment. Both prebiotic levels significantly reduced feed intake & reduced feed conversion and increased weight gain in comparison with control treatment (P<0.05). Both prebiotic levels significantly decreased the hemoglobin, red blood cells, protein and hematocrit in birds were induced ascites (P<0.05). Both prebiotic levels significantly decreased ascites and RV / TV index (P<0.05). The results of intestinal morphology indicated that the weight of the intestines (dissected from below the gizzard to the ileal cecal junction) was not significantly different between the experimental groups. in Conclusion, it is possible to successfully use a 2gg/kg diet of prebiotic (Fermacto) in broiler chickens as an effective factor in reducing functional problems and ascites mortality.
Mokhtar Fathi; Shahriar Saeidian; Kobra Varmaghani
Abstract
A study was conducted to evaluate the antioxidant effects of selenium nanoparticles and sodium selenite on the antioxidant status, enzymatic activity and plasma lipid parameters of Pullet Chickswith 1050 birds in 7 treatments for 6 weeks. The treatments included three levels of 0.15, 0.3 and 0.6 (ppm) ...
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A study was conducted to evaluate the antioxidant effects of selenium nanoparticles and sodium selenite on the antioxidant status, enzymatic activity and plasma lipid parameters of Pullet Chickswith 1050 birds in 7 treatments for 6 weeks. The treatments included three levels of 0.15, 0.3 and 0.6 (ppm) sodium selenite and nano selenium. The antioxidant indices measured in the heart, liver and plasma include glutathione peroxidase (GPx), malondialdehyde (MDA) and total antioxidant capacity (TAS). Activity of alanine aminotransferase (ALT) enzymes, aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were measured and lipid parameters including total triglyceride, total cholesterol and plasma LDL were determined. The results showed that sodium selenite and selenium nanoparticles significantly increased the activity of glutathione peroxidase and reduced levels of malondialdehyde in the plasma, heart and liver. The highest antioxidant effect was related to 0.6 ppm sodium selenium and 0.3 ppm selenium nanoparticles. There was no significant effect on total antioxidant capacity of plasma, heart and liver in experimental group (P<0.05) .There was no significant effect of experimental treatments on plasma enzyme activity (P<0.05). Sodium selenite and selenium nanoparticles at levels of 0.3 and 0.6 ppm significantly decreased triglyceride, cholesterol and LDL in the plasma of birds (P<0.05). Conclusion 0.3 ppm of selenium nanoparticles and 0.6 ppm of Sodium selenitecan be successfully used to improve the antioxidant status and reduce plasma bad fats.
M Fathi; T Tanha
Volume 3, Issue 1 , January 2015, , Pages 1-6
Abstract
Two hundred forty 1-d-old Ross 308 male broiler chicks were randomly allocated into 3 groups with 4 replicates. From d 14, the diets were supplemented with CoQ10 at levels of 0, 20, and 40 mg/kg, respectively. From d 14 to 42, all the chicks were exposed to low ambient temperature (10 to 15°C) to ...
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Two hundred forty 1-d-old Ross 308 male broiler chicks were randomly allocated into 3 groups with 4 replicates. From d 14, the diets were supplemented with CoQ10 at levels of 0, 20, and 40 mg/kg, respectively. From d 14 to 42, all the chicks were exposed to low ambient temperature (10 to 15°C) to induce Ascites. Mortality was inspected to determine cause of death and diagnosis of PHS. For evaluation of antioxidant status, the parameters that were determined in plasma and liver. (GPX, SOD, TAS and MDA). Sampling of blood and liver tissue were determined at d 21 and 42. At end of the experiment (wk 6), 2 chicks from each replicate were randomly selected and slaughtered. The heart was removed; the right ventricle was dissected away from the left ventricle and septum then RV/TV calculated. The results showed that mortality due to Ascites was reduced by CoQ10 supplementation (P10 compared with the control, but no significant changes were observed on SOD activity and TAS. RV/TV was significantly decreased by 40 mg/kg CoQ10 supplementation (PP>0.05).
mokhtar fathi; T. Tanha
Volume 1, Issue 1 , October 2012, , Pages 69-80
Abstract
160 one-day-old male broiler chicks (Ross308) in 2 groups of 4 replicates were studied in each group20 chicks for each replicate were used. One group of these birds was rose in normal temperature (NT treatment) and the other group was raised in cold temperature induce PHS and heart failure ...
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160 one-day-old male broiler chicks (Ross308) in 2 groups of 4 replicates were studied in each group20 chicks for each replicate were used. One group of these birds was rose in normal temperature (NT treatment) and the other group was raised in cold temperature induce PHS and heart failure (CT treatment). Glutathione peroxidase (GPX), superoxide dismutase SOD (, total antioxidant status (TAS) and malondialdehyde (MDA) content of both plasma and liver were determined at days 21 and 42 At the end of the experiment (day42), 2 chicks from each replicate were randomly selected and slaughtered. The heart was removed and the right ventricle was dissected away. The ratio of right ventricle weight to total ventricle weight (RV/TV) was calculated. Results showed that MDA content in plasma and liver of CT birds was greater than that of NT birds at day 21 and 42. GPX activity in plasma and liver of CT birds at day 21&42 and SOD activity in plasma at day 42 were lower than that of NT birds. Furthermore, birds of NT treatment had a higher plasma TAS (P<0.05) at both ages. Moreover, RV/TV ratio at day 42 and mortality in total period were significantly higher in CT birds.
